Laboratory of Synthetic Genome Biology
Developing biological engineering approaches to understand epigenetics and genome organization in C. elegans

Froekjaer-Jensen et al (2010)

Targeted gene deletions in C. elegans using transposon excision.

Froekjaer-Jensen et al. (2010). Targeted gene deletions in C. elegans using transposon excision. Nature Methods. ​
Frøkjaer-Jensen C, Davis MW, Hollopeter G, Taylor J, Harris TW, Nix P, Lofgren R, Prestgard-Duke M, Bastiani M, Moerman DG, Jorgensen EM.
Transgenesis, Mos1, transposon, targeted deletion, C. elegans, Jorgensen lab, University of Utah
We developed a method, MosDEL, to generate targeted knockouts of genes in Caenorhabditis elegans by injection. We generated a double-strand break by mobilizing a Mos1 transposon adjacent to the region to be deleted; the double-stranded break is repaired using injected DNA as a template. Repair can delete up to 25 kb of DNA and simultaneously insert a positive selection marker.